Rabbit anti-Citron Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-303A maps to a region between residue 1925 and 1975 of human citron (rho-interacting, serine/threonine kinase 21) using the numbering given in entry NP_009105.1 (GeneID 11113).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Citron immobilized on solid support.
The epitope recognized by A302-303A-T maps to a region between residue 1925 and 1975 of human citron (rho-interacting, serine/threonine kinase 21) using the numbering given in entry NP_009105.1 (GeneID 11113).
Additional Product Information
Citron is a dual specificity serine/threonine protein kinase and a putative RHO/RAC effector that binds to the GTP-bound forms of RHO and RAC1. It is required for KIF14 localization to the central spindle and midbody and may play a role in cytokinesis. Citron also plays an important role in the regulation of cytokinesis and the development of the central nervous system [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O14578].
Alternate Names
CITK; citron (rho-interacting, serine/threonine kinase 21); citron Rho-interacting kinase; CRIK; MCPH17; serine/threonine kinase 21; serine/threonine-protein kinase 21; STK21
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.