/ Antibodies & Antisera / Primary Antibodies / Rabbit anti-Cortactin Antibody
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Primary Antibodies  

Rabbit anti-Cortactin Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
Cortactin
Reactivity:
Human
Applications:
IHC, IP, WB
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 450 and 500
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (200 µg/ml)

10 µl (200 µg/ml)

$121.00 $433.00
Qty:
Bulk Request

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
200 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Request Formulation Change
Country of Origin
USA

Additional Product Information

Cortactin has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. The aberrant regulation of cortactin contributes to tumor cell invasion and metastasis [taken from NCBI Entrez Gene (GeneID: 2017)].

2017
CTTN
cortactin
Q14247
Src substrate cortactin

Alternate Names

1110020L01Rik; amplaxin; EMS1; ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 src substrate)); epididymis secretory sperm binding protein; oncogene EMS1; src substrate cortactin

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.