Rabbit anti-COX4 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-899A maps to a region between residue 25 and 75 of human cytochrome c oxidase subunit IV isoform 1 using the numbering given in entry NP_001852.1 (GeneID 1327).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to COX4 immobilized on solid support.
The epitope recognized by A301-899A-T maps to a region between residue 25 and 75 of human cytochrome c oxidase subunit IV isoform 1 using the numbering given in entry NP_001852.1 (GeneID 1327).
Additional Product Information
COX4 (cytochrome c oxidase, subunit IV isoform 1) is one of 13 subunits of the multiprotein COX (cytochrome oxidase) complex which functions as the terminal enzyme in the mitochondrial respiratory chain.
Alternate Names
COX IV-1; COX4; COX4-1; COXIV; COXIV-1; cytochrome c oxidase polypeptide IV; cytochrome c oxidase subunit 4 isoform 1, mitochondrial; cytochrome c oxidase subunit IV; Cytochrome c oxidase subunit IV isoform 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.