Rabbit anti-COX5A Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-577A maps to a region between residue 100 to 150 of human Cytochrome c oxidase subunit 5A, mitochondrial using the numbering given in entry P20674.2 (GeneID 9377).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to COX5A immobilized on solid support.
The epitope recognized by A305-577A-T maps to a region between residue 100 to 150 of human Cytochrome c oxidase subunit 5A, mitochondrial using the numbering given in entry P20674.2 (GeneID 9377).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. COX5A is the nuclear-encoded subunit Va of the human mitochondrial respiratory chain enzyme [taken from NCBI Entrez Gene (Gene ID: 9377)].
Alternate Names
COX; COX-VA; cytochrome c oxidase polypeptide Va; cytochrome c oxidase polypeptide, mitochondrial; cytochrome c oxidase subunit 5A, mitochondrial; cytochrome c oxidase subunit Va; mitochondrial cytochrome c oxidase subunit Va; VA
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.