Rabbit anti-CPSF73 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-091A maps to a region between residue 634 and 684 of human cleavage and polyadenylation specific factor 3, 73kDa using the numbering given in entry NP_057291.1 (GeneID 51692).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CPSF73 immobilized on solid support.
The epitope recognized by A301-091A-T maps to a region between residue 634 and 684 of human cleavage and polyadenylation specific factor 3, 73kDa using the numbering given in entry NP_057291.1 (GeneID 51692).
Additional Product Information
CPSF73 is the 73 kDa subunit of the cleavage and polyadenylation specificity factor (CPSF) complex that functions in pre-mRNA-3’-end formation. CPSF73 has been implicated as the endonuclease component of the CPSF complex for pre-mRNA-3’ end processing.
Alternate Names
cleavage and polyadenylation specific factor 3, 73kDa; Cleavage and polyadenylation specificity factor 73 kDa subunit; cleavage and polyadenylation specificity factor subunit 3; CPSF 73 kDa subunit; CPSF73; CPSF-73; mRNA 3'-end-processing endonuclease CPSF-73
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.