Rabbit anti-CTDP1/FCP1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-171A maps to a region between residue 500 and 550 of human CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) phosphatase, subunit 1 (TFIIF-associating CTD phosphatase 1) using the numbering given in entry NP_004706.3 (GeneID 9150).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CTDP1/FCP1 immobilized on solid support.
The epitope recognized by A301-171A-T maps to a region between residue 500 and 550 of human CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) phosphatase, subunit 1 (TFIIF-associating CTD phosphatase 1) using the numbering given in entry NP_004706.3 (GeneID 9150).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
CTDP1/FCP1 is a phosphatase that is responsible for the dephosphorylation of RNA polymerase II (RNA Pol II) C-terminal domain (CTD) and is a critical regulator of RNA Pol II function in the steps of transcription.
Alternate Names
CCFDN; CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) phosphatase, subunit 1; CTD of POLR2A, phosphatase of, subunit 1; FCP1; RNA polymerase II subunit A C-terminal domain phosphatase; serine phosphatase FCP1a; TFIIF-associating CTD phosphatase; TFIIF-associating CTD phosphatase 1; transcription factor IIF-associating CTD phosphatase 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.