Rabbit anti-Cyclin B2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-509A-T maps to a region between residue 1 to 50 of human Cyclin B2 using the numbering given in entry NP_004692.1 (GeneID 9133).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Cyclin B2 immobilized on solid support.
The epitope recognized by A304-509A maps to a region between residue 1 to 50 of human Cyclin B2 using the numbering given in entry NP_004692.1 (GeneID 9133).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control [taken from NCBI Entrez Gene (Gene ID: 9133)].
Alternate Names
G2/mitotic-specific cyclin-B2; HsT17299
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.