Rabbit anti-Cyclin T1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-499A maps to a region between residue 625 and 675 of human Cyclin T1 using the numbering given in entry NP_001231.2 (GeneID 904).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Cyclin T1 immobilized on solid support.
The epitope recognized by A303-499A-T maps to a region between residue 625 and 675 of human Cyclin T1 using the numbering given in entry NP_001231.2 (GeneID 904).
Additional Product Information
Cyclin T1 is a regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, Cyclin T1 binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O60563].
Alternate Names
CCNT; CDK9-associated C-type protein; cyclin C-related protein; Cyclin-T; cyclin-T1; CYCT1; HIVE1; human immunodeficiency virus type 1 (HIV-1) expression (elevated) 1; MLLT10/CCNT1 fusion
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.