Rabbit anti-Cytokeratin 20 Recombinant Monoclonal Antibody [BLR105H]
Product Details
Specifications
Request Formulation Change Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
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Immunogen was a peptide representing a region between residue 250 and 300 of human keratin, type I cytoskeletal 20 using the numbering given in entry NP_061883.1 (Gene ID 54474).
Additional Product Information
Cytokeratin 20 is a member of the keratin family of intermediate filament proteins responsible for the structural integrity of epithelial cells. Cytokeratin 20 is a type I cytokeratin, acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. [taken from NCBI Entrez Gene (Gene ID: 54474)].
Alternate Names
CD20; CK20; CK-20; cytokeratin 20; Cytokeratin-20; K20; keratin 20, type I; keratin, type I cytoskeletal 20; Keratin-20; KRT21; protein IT
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.