Rabbit anti-DCBLD2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-454A maps to a region between residue 725 to 775 of human Discoidin, CUB and LCCL Domain Containing 2 using the numbering given in entry NP_563615.3 (GeneID 131566).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to DCBLD2 immobilized on solid support.
The epitope recognized by A304-454A-T maps to a region between residue 725 to 775 of human Discoidin, CUB and LCCL Domain Containing 2 using the numbering given in entry NP_563615.3 (GeneID 131566).
Additional Product Information
Discoidin, CUB and LCCL domain-containing protein 2 (DCBLD2) is highly expressed in testis, heart, skeletal muscle and also in cultured vascular smooth muscle cells [Taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96PD2].
Alternate Names
1700055P21Rik; CLCP1; coagulation factor V/VIII-homology domains protein 1; CUB, LCCL and coagulation factor V/VIII-homology domains protein 1; discoidin, CUB and LCCL domain-containing protein 2; endothelial and smooth muscle cell-derived neuropilin-like protein; ESDN
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.