Rabbit anti-DCP1A Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-591A maps to a region between residue 475 and 525 of human DCP1 Decapping Enzyme Homolog A using the numbering given in entry NP_060873.4 (GeneID 55802).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to DCP1A immobilized on solid support.
The epitope recognized by A303-591A-T maps to a region between residue 475 and 525 of human DCP1 Decapping Enzyme Homolog A using the numbering given in entry NP_060873.4 (GeneID 55802).
Additional Product Information
mRNA-decapping enzyme 1A (DCP1A) is necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. DCP1A removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. DCP1A also contributes to the transactivation of target genes after stimulation by TGFB1 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q9NPI6].
Alternate Names
DCP1 decapping enzyme homolog A; DCP1 decapping enzyme-like protein A; decapping enzyme hDcp1a; HSA275986; mRNA-decapping enzyme 1A; Nbla00360; putative protein product of Nbla00360; Smad4-interacting transcriptional co-activator; SMAD4IP1; SMIF; transcription factor SMIF
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.