Rabbit anti-DCP1B Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-640A-T maps to a region between residue 200 and 250 of human DCP1 Decapping Enzyme Homolog B using the numbering given in entry NP_689853.3 (GeneID 196513).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to DCP1B immobilized on solid support.
The epitope recognized by A303-640A maps to a region between residue 200 and 250 of human DCP1 Decapping Enzyme Homolog B using the numbering given in entry NP_689853.3 (GeneID 196513).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
mRNA-decapping enzyme 1B (DCP1B) may play a role in the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. It may remove the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP (by similarity) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q8IZD4].
Alternate Names
DCP1; DCP1 decapping enzyme homolog B; decapping enzyme hDcp1b; mRNA-decapping enzyme 1B
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.