Rabbit anti-DDX28 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-762A maps to a region between residue 500 and the C-terminus (residue 540) of human DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 28 using the numbering given in entry NP_060850.1 (GeneID 55794).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to DDX28 immobilized on solid support.
The epitope recognized by A300-762A-T maps to a region between residue 500 and the C-terminus (residue 540) of human DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 28 using the numbering given in entry NP_060850.1 (GeneID 55794).
Additional Product Information
DDX28 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DDX28 is nuclear shuttling protein and has been found to be localized to the nucleus and mitochondria.
Alternate Names
DEAD (Asp-Glu-Ala-Asp) box polypeptide 28; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 28; MDDX28; mitochondrial DEAD box protein 28; mitochondrial DEAD-box polypeptide 28; probable ATP-dependent RNA helicase DDX28
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.