Rabbit anti-DDX6 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-461A maps to a region between residues 425 and 483 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 using the numbering given Swiss-Prot entry NP_004388.2 (GeneID 1656).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to DDX6 immobilized on solid support.
The epitope recognized by A300-461A-T maps to a region between residues 425 and 483 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 using the numbering given Swiss-Prot entry NP_004388.2 (GeneID 1656).
Additional Product Information
DDX6 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DDX6 has been observed to positively regulate growth by modulating gene expression at the translational level.
Alternate Names
ATP-dependent RNA helicase p54; DEAD (Asp-Glu-Ala-Asp) box helicase 6; DEAD (Asp-Glu-Ala-Asp) box polypeptide 6; DEAD box protein 6; DEAD box-6; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD); HLR2; oncogene RCK; P54; probable ATP-dependent RNA helicase DDX6; RCK
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.