Rabbit anti-DGCR14 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-566A maps to a region between residue 426 to 476 of human DiGeorge Syndrome Critical Region Gene 14 using the numbering given in entry NP_073210.1 (GeneID 8220).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
The gene encoding DGCR14 (DiGeorge syndrome critical region gene 14) is located within the minimal DGS critical region (MDGCR) thought to contain the gene(s) responsible for a group of developmental disorders. These disorders include DiGeorge syndrome, velocardiofacial syndrome, conotruncal anomaly face syndrome, and some familial or sporadic conotruncal cardiac defects which have been associated with microdeletion of 22q11.2. The DGCR14 protein may be a component of C complex spliceosomes, and the orthologous protein in the mouse localizes to the nucleus [taken from NCBI Entrez Gene (Gene ID: 8220)].
Alternate Names
bis1; DGCR13; DGCR14; DGSH; DGS-H; DGSI; DGS-I; DiGeorge syndrome critical region 13; DiGeorge syndrome critical region 14; DiGeorge syndrome critical region gene 13; DiGeorge syndrome critical region gene 14; DiGeorge syndrome critical region gene DGSI; DiGeorge syndrome gene H; DiGeorge syndrome gene I; diGeorge syndrome protein H; ES2; Es2el; ESS-2; Protein DGCR13; protein DGCR14; Protein ES2; splicing factor ESS-2 homolog
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.