Rabbit anti-DHX8 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-624A maps to a region between residues 325 and 375 of human DEAH (Asp-Glu-Ala-His) Box Polypeptide 8 using the numbering given in entry NP_004932.1 (GeneID 1659).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to DHX8 immobilized on solid support.
The epitope recognized by A300-624A-T maps to a region between residues 325 and 375 of human DEAH (Asp-Glu-Ala-His) Box Polypeptide 8 using the numbering given in entry NP_004932.1 (GeneID 1659).
Additional Product Information
DHX8 is a member of the DEAH box family of proteins that possesses several conserved motifs which include the highly conserved DEAH (Asp-Glu-Ala-His) amino acid sequence motif. The major activity of DEAH box proteins is to function as ATP-dependent RNA helicases. RNA helicases play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DHX8 is the human ortholog of the yeast Prp22 protein. DHX8 has been demonstrated to function in the release of RNA from the splicesome and the nuclear export of spliced mRNA.
Alternate Names
ATP-dependent RNA helicase DHX8; DDX8; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 8 (RNA helicase); DEAH (Asp-Glu-Ala-His) box polypeptide 8; DEAH box protein 8; Dhr2; HRH1; PRP22; PRPF22; RNA helicase HRH1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.