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Rabbit anti-DIAPH3 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
DIAPH3
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 1 and 50
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to DIAPH3 immobilized on solid support.

The epitope recognized by A305-164A maps to a region between residue 1 to 50 of human Protein diaphanous homolog 3 using the numbering given in entry Q9NSV4.4 (GeneID 81624).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to DIAPH3 immobilized on solid support.

The epitope recognized by A305-164A-T maps to a region between residue 1 to 50 of human Protein diaphanous homolog 3 using the numbering given in entry Q9NSV4.4 (GeneID 81624).
Country of Origin
USA

Additional Product Information

DIAPH3 encodes for protein diaphanous homolog 3, a member of the diaphanous subfamily of the formin family. Members of this family are involved in actin remodeling and regulate cell movement and adhesion. Mutations in DIAPH3 are associated with autosomal dominant auditory neuropathy 1 [taken from NCBI Entrez Gene (Gene ID: 81624)].

81624
DIAPH3
diaphanous related formin 3
Q9NSV4
Protein diaphanous homolog 3

Alternate Names

AN; AUNA1; DIA2; diap3; diaphanous homolog 3; Diaphanous-related formin-3; DRF3; mDia2; NSDAN; protein diaphanous homolog 3

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.