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Rabbit anti-Diaphanous 2 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
Diaphanous 2
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 50 and 100
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to Diaphanous 2 immobilized on solid support.

The epitope recognized by A300-079A maps to a region between residues 50 and 100 of human Diaphanous Homolog 2 using the numbering given in entry NP_006720.1 (GeneID 1730).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to Diaphanous 2 immobilized on solid support.

The epitope recognized by A300-079A-T maps to a region between residues 50 and 100 of human Diaphanous Homolog 2 using the numbering given in entry NP_006720.1 (GeneID 1730).
Country of Origin
USA

Additional Product Information

Diaphanous 2 is a member of the formin family of proteins that is responsible for actin polymerization and nucleation activity and the formation of linear, unbranched actin cables and stress fibers. Diaphanous 1 is a target of the small GTPase, Rho. RhoB and diaphanous 2 have been shown to interact on endosomes and control actin dynamics involved in vesicle trafficking.

1730
DIAPH2
diaphanous related formin 2
O60879
Protein diaphanous homolog 2

Alternate Names

DIA; DIA2; diaphanous homolog 2; Diaphanous-related formin-2; diaphorase-2; DRF2; POF; POF2; POF2A; protein diaphanous homolog 2

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).