Rabbit anti-DNA Helicase B Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-686A maps to a region between residue 1037 to 1087 of human DNA Helicase B using the numbering given in entry NP_387467.2 (GeneID 92797).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to DNA Helicase B immobilized on solid support.
The epitope recognized by A304-686A-T maps to a region between residue 1037 to 1087 of human DNA Helicase B using the numbering given in entry NP_387467.2 (GeneID 92797).
Additional Product Information
DNA Helicase B unwinds duplex DNA with 5'-3' polarity. It has single-strand DNA-dependent ATPase and DNA helicase activities. DNA helicase B prefers ATP and dATP as substrates. During S phase, it may facilitate cellular recovery from replication stress [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q8NG08].
Alternate Names
DHB; DNA helicase B; hDHB; helicase (DNA) B
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.