Rabbit anti-DNAJB1/Hsp40 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-245A maps to a region between residue 290 to 340 of human DnaJ homolog subfamily B member 1 using the numbering given in entry P25685.4 (GeneID 3337).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to DNAJB1/Hsp40 immobilized on solid support.
The epitope recognized by A305-245A-T maps to a region between residue 290 to 340 of human DnaJ homolog subfamily B member 1 using the numbering given in entry P25685.4 (GeneID 3337).
Additional Product Information
DNAJB1 Interacts with HSP70 and can stimulate its ATPase activitY. DNAJB1 stimulates the association between HSC70 and HIP [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P25685].
Alternate Names
DnaJ (Hsp40) homolog, subfamily B, member 1; dnaJ homolog subfamily B member 1; dnaJ protein homolog 1; Hdj1; hDj-1; heat shock 40 kDa protein 1; Heat shock protein 40; Hsp40; HSPF1; human DnaJ protein 1; radial spoke 16 homolog B; RSPH16B; Sis1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.