Rabbit anti-DOCK7 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-357A maps to a region between residue 175 and 225 of human Dedicator of Cytokinesis 7 using the numbering given in entry Q96N67.4 (GeneID 85440).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to DOCK7 immobilized on solid support.
The epitope recognized by A303-357A-T maps to a region between residue 175 and 225 of human Dedicator of Cytokinesis 7 using the numbering given in entry Q96N67.4 (GeneID 85440).
Additional Product Information
Dedicator of cytokinesis protein 7 (DOCK7) functions as a guanine nucleotide exchange factor (GEF), which activates Rac1 and Rac3 Rho small GTPases by exchanging bound GDP for free GTP. DOCK7 does not have a GEF activity for CDC42. It is required for STMN1 'Ser-15' phosphorylation during axon formation and consequently for neuronal polarization [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96N67].
Alternate Names
dedicator of cytokinesis protein 7; EIEE23; ZIR2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.