Rabbit anti-DOT1L IHC Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by IHC-00494 maps to a region between residue1000 and 1050 of human DOT1-Like using the numbering given in entry NP_115871.1 (GeneID 84444).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to DOT1L immobilized on solid support.
The epitope recognized by IHC-00494-T maps to a region between residue1000 and 1050 of human DOT1-Like using the numbering given in entry NP_115871.1 (GeneID 84444).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
DOT1L is a histone methyltransferase that methylates nucleosomal histone H3 on lysine 79. In contrast to other lysine histone methyltransferase, DOT1L does not contain a SET domain, a domain shown to be necessary for methyltransferase activity.
Alternate Names
DOT1; DOT1 like histone H3K79 methyltransferase; DOT1-like histone methyltransferase; DOT1-like protein; DOT1-like, histone H3 methyltransferase; H3-K79-HMTase; histone H3-K79 methyltransferase; histone methyltransferase DOT1L; histone-lysine N-methyltransferase, H3 lysine-79 specific; KMT4; lysine N-methyltransferase 4
Applications
Epitope exposure is recommended.
Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.