Rabbit anti-DTD1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-671A maps to a region between residue 159 to 209 of human D-Tyrosyl-tRNA Deacylase 1 using the numbering given in entry NP_543010.3 (GeneID 92675).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to DTD1 immobilized on solid support.
The epitope recognized by A304-671A-T maps to a region between residue 159 to 209 of human D-Tyrosyl-tRNA Deacylase 1 using the numbering given in entry NP_543010.3 (GeneID 92675).
Additional Product Information
D-tyrosyl-tRNA(Tyr) deacylase 1 (DTD1) is an ATPase involved in DNA replication. It may facilitate loading of CDC45 onto pre-replication complexes. DTD1 may hydrolyze D-tyrosyl-tRNA(Tyr) into D-tyrosine and free tRNA(Tyr), a possible defense mechanism against a harmful effect of D-tyrosine [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q8TEA8].
Alternate Names
C20orf88; D-aminoacyl-tRNA deacylase 1; DNA-unwinding element-binding protein B; DTD; D-tyrosyl-tRNA deacylase 1 homolog; D-tyrosyl-tRNA(Tyr) deacylase 1; DUEB; DUE-B; gly-tRNA(Ala) deacylase; HARS2; histidyl-tRNA synthase-related; histidyl-tRNA synthetase 2; pqn-68
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.