Rabbit anti-E4BP4 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-606A maps to a region between residue 412 and 462 of human E4 promoter binding-protein 4 using the numbering given in entry NP_005375.2 (GeneID 4783).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to E4BP4 immobilized on solid support.
The epitope recognized by A302-606A-T maps to a region between residue 412 and 462 of human E4 promoter binding-protein 4 using the numbering given in entry NP_005375.2 (GeneID 4783).
Additional Product Information
E4 promoter-binding protein 4 (E4BP4) functions as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. E4BP4 activates transcription from the interleukin-3 promoter in T-cells and is a component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q16649].
Alternate Names
E4 promoter-binding protein 4; E4BP4; IL3BP1; interleukin-3 promoter transcriptional activator; interleukin-3-binding protein 1; NFIL3A; NF-IL3A; nuclear factor interleukin-3-regulated protein; transcriptional activator NF-IL3A
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.