Rabbit anti-E4F1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-832A maps to a region between residue 725 and the C-terminus (residue 784) of human E4F1 (p120E4F) using the numbering given in entry NP_004415.1 (GeneID 1877).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to E4F1 immobilized on solid support.
The epitope recognized by A300-832A-T maps to a region between residue 725 and the C-terminus (residue 784) of human E4F1 (p120E4F) using the numbering given in entry NP_004415.1 (GeneID 1877).
Additional Product Information
E4F1 is a Kruppel-like family member identified as a cellular protein activated by the viral E1A oncoprotein. E4F1 can function as an activator or repressor of transcription to control embryonic and somatic cell proliferation and survival. E4F1 has also been identified as an atypical ubiquitin E3 ligase for p53.
Alternate Names
E4F; E4F transcription factor 1; p120E4F; p50E4F; putative E3 ubiquitin-protein ligase E4F1; RING-type E3 ubiquitin transferase E4F1; transcription factor E4F; transcription factor E4F1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.