Rabbit anti-E6AP Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-351A maps to a region between residue 75 and 125 of human Ubiquitin Protein Ligase E3A using the numbering given in entry NP_000453.2 (GeneID 7337).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to E6AP immobilized on solid support.
The epitope recognized by A300-351A-T maps to a region between residue 75 and 125 of human Ubiquitin Protein Ligase E3A using the numbering given in entry NP_000453.2 (GeneID 7337).
Additional Product Information
E6AP is an E3 ubiquitin ligase. Inheritance of a mutant maternal copy of this gene results in Angelman syndrome (AS) which is characterized by severe mental retardation, seizures, ataxia, and EEG abnormalities. E6AP interacts with the type 16 and 18 human papillomavirus E6 oncoprotein which results in the targeting of p53 for degradation via the ubiquitin-proteasome system.
Alternate Names
ANCR; AS; CTCL tumor antigen se37-2; E6-AP; E6AP ubiquitin-protein ligase; EPVE6AP; HECT-type ubiquitin transferase E3A; HPVE6A; human papilloma virus E6-associated protein; human papillomavirus E6-associated protein; oncogenic protein-associated protein E6-AP; renal carcinoma antigen NY-REN-54; ubiquitin-protein ligase E3A
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.