Rabbit anti-EB1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-333A maps to a region between residue 245 and 268 of human End-binding protein 1 using the numbering given in entry NP_036457.1 (GeneID 22919).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to EB1 immobilized on solid support.
The epitope recognized by A302-333A-T maps to a region between residue 245 and 268 of human End-binding protein 1 using the numbering given in entry NP_036457.1 (GeneID 22919).
Additional Product Information
End –binding protein 1 (EB1) is a member of the RP/EB family and was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. EB1 also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that EB1 is involved in the regulation of microtubule structures and chromosome stability. [taken from NCBI GeneID:22919].
Alternate Names
adenomatous polyposis coli-binding protein EB1; APC-binding protein EB1; EB1; end-binding protein 1; microtubule-associated protein RP/EB family member 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.