Rabbit anti-EG5/KIF11 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-075A maps to a region between residue 1006 and the C-terminus (residue 1056) of human kinesin-related motor protein Eg5 (kinesin family member 11) using the numbering given in entry NP_004514.2 (GeneID 3832).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to EG5/KIF11 immobilized on solid support.
The epitope recognized by A301-076A-T maps to a region between residue 1006 and the C-terminus (residue 1056) of human kinesin-related motor protein Eg5 (kinesin family member 11) using the numbering given in entry NP_004514.2 (GeneID 3832).
Additional Product Information
The kinesin superfamily of proteins consists of forty five KIF motor proteins that function in intracellular transport along microtubules. Kinesin activity has been linked to various cellular functions such as vesicle transport, mitotic spindle formation, chromosome segregation, and cytokinesis. Structurally, all kinesins contain a motor domain with microtubule and nucleotide binding sites that utilize ATP to target cargo along microtubule filaments. EG5/KIF11 plays a role in spindle-pole formation and chromosome positioning and separation.
Alternate Names
EG5; HKSP; kinesin-like protein 1; kinesin-like protein KIF11; kinesin-like spindle protein HKSP; kinesin-related motor protein Eg5; KNSL1; MCLMR; thyroid receptor-interacting protein 5; TR-interacting protein 5; TRIP5; TRIP-5
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.