Rabbit anti-eIF2A Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
Eukaryotic initiation factor 2A (eIF2A) directs binding of the intiator methionyl-tRNA [Met-tRNA(i)] to 40 S ribosomal subunits in a codon dependent manner. It is a 65 kDa protein that was originally believed to be the functional homolog of prokaryotic IF2. eIF2A displays functional qualities similar to eIF2 which exists as complex of 3 subunits (eIF2-alpha, beta, and gamma); however comparison of eIF2A and eIF2 has shown that these two intitiation factors also display distinct functional abilities.
Alternate Names
65 kDa eukaryotic translation initiation factor 2A; CDA02; EIF-2A; eukaryotic translation initiation factor 2A; eukaryotic translation initiation factor 2A, 65kDa; MST089; MSTP004; MSTP089
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.