Rabbit anti-eIF3A/eIF3S10 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-003A maps to a region between residue 860 and 910 of human translation initiation factor 3 subunit A (eukaryotic translation initiation factor 3, subunit 10) using the numbering given in entry NP_003741.1 (GeneID 8661).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to eIF3A/eIF3S10 immobilized on solid support.
The epitope recognized by A302-003A-T maps to a region between residue 860 and 910 of human translation initiation factor 3 subunit A (eukaryotic translation initiation factor 3, subunit 10) using the numbering given in entry NP_003741.1 (GeneID 8661).
Additional Product Information
Eukaryotic initiation factor 3 subunit A (eIF3A) is one of at least 13 non-identical protein subunits of eukaryotic initiation factor 3 (eIF3). eIF3 is the largest eIF (~650 kDa) and functions to facilitate binding of the 40S ribosomal subunit to the 5’-end of cellular mRNAs near the cap structure (m7GpppN). eIF3A is also known as EIF3S10 (eukaryotic initiation factor 3 subunit 7). eIF3A is the largest subunit of the eIF3 complex. eIF3A expression is regulated through the cell cycle. It’s expression peaks in S-phase and it is proposed to play a role in regulating the translation of mRNAs involved in the regulation of cell cycle progression and proliferation.
Alternate Names
centrosomin homolog; cytoplasmic protein p167; EIF3; eIF3 p167; eIF3 p180; eIF3 p185; EIF3, p180 subunit; eIF3a; eIF3-p170; EIF3S10; eIF-3-theta; eIF3-theta; eukaryotic translation initiation factor 3 subunit 10; eukaryotic translation initiation factor 3 subunit A; eukaryotic translation initiation factor 3, subunit 10 (theta, 150/170kD); eukaryotic translation initiation factor 3, subunit 10 (theta, 170kD); eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa; eukaryotic translation initiation factor 3, subunit 10, 170kD; P167; p180; p185; TIF32
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.