Rabbit anti-eIF3E Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-984A maps to a region between residue 1 and 50 of human Eukaryotic Translation Initiation Factor 3, Subunit E using the numbering given in entry NP_001559.1 (GeneID 3646).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to eIF3E immobilized on solid support.
The epitope recognized by A302-984A-T maps to a region between residue 1 and 50 of human Eukaryotic Translation Initiation Factor 3, Subunit E using the numbering given in entry NP_001559.1 (GeneID 3646).
Additional Product Information
eIF3E (eukaryotic translation initiation factor 3, subunit E) is also known as INT6 (viral integration site protein INT-6 homolog). eIF3e is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex which is required for several steps in the initiation of protein synthesis.
Alternate Names
eIF-3 p48; eIF3e; eIF3-p46; EIF3-P48; EIF3S6; eukaryotic translation initiation factor 3 subunit 6; eukaryotic translation initiation factor 3 subunit E; eukaryotic translation initiation factor 3 subunit E isoform 2 transcript; eukaryotic translation initiation factor 3, subunit 6 (48kD); eukaryotic translation initiation factor 3, subunit 6 48kDa; INT6; mammary tumor-associated protein INT6; murine mammary tumor integration site 6 (oncogene homolog); viral integration site protein INT-6 homolog
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.