/ Antibodies & Antisera / Primary Antibodies / Rabbit anti-eIF3G/EIF3S4 Antibody
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Primary Antibodies  

Rabbit anti-eIF3G/EIF3S4 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
eIF3G/EIF3S4
Reactivity:
Human
Applications:
WB
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 100 and 150
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (200 µg/ml)

10 µl (200 µg/ml)

$131.00 $464.00
Qty:
Bulk Request

Product Details

Specifications

Verified Reactivity
Human
Presumed Reactivity
Mouse, Bovine, Rat
Antigen Species
Human
Concentration
200 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to eIF3G/EIF3S4 immobilized on solid support.

The epitope recognized by A301-756A maps to a region between residue 100 and 150 of human eukaryotic translation initiation factor 3, subunit G (eukaryotic translation initiation factor 3, subunit 4) using the numbering given in entry NP_003746.2 (GeneID 8666).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to eIF3G/EIF3S4 immobilized on solid support.

The epitope recognized by A301-756A-T maps to a region between residue 100 and 150 of human eukaryotic translation initiation factor 3, subunit G (eukaryotic translation initiation factor 3, subunit 4) using the numbering given in entry NP_003746.2 (GeneID 8666).
Country of Origin
USA

Additional Product Information

Eukaryotic initiation factor 3 subunit G (eIF3G) is one of at least 13 non-identical protein subunits of eukaryotic initiation factor 3 (eIF3). eIF3 is the largest eIF (~650 kDa) and functions to facilitate binding of the 40S ribosomal subunit to the 5’-end of cellular mRNAs near the cap structure (m7GpppN). eIF3G has been shown to directly interact with AIF (apoptosis inducing factor), a protein that plays a role in caspase-independent apoptosis. AIF is able to inhibit protein synthesis via its interaction with eIF3g.

8666
EIF3G
eukaryotic translation initiation factor 3 subunit G
O75821
Eukaryotic translation initiation factor 3 subunit G

Alternate Names

eIF3 p42; eIF3 p44; eIF-3 RNA-binding subunit; eIF-3-delta; eIF3-delta; eIF3g; EIF3-P42; eIF3-p44; EIF3S4; eukaryotic translation initiation factor 3 RNA-binding subunit; Eukaryotic translation initiation factor 3 subunit 4; eukaryotic translation initiation factor 3 subunit G; eukaryotic translation initiation factor 3 subunit p42; eukaryotic translation initiation factor 3, subunit 4 delta, 44kDa

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).