Rabbit anti-EIF3L Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-753A maps to a region between residue 1 to 50 of human Eukaryotic Translation Initiation Factor 3 Subunit L using the numbering given in entry Q9Y262.1 (GeneID 51386).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to EIF3L immobilized on solid support.
The epitope recognized by A304-753A-T maps to a region between residue 1 to 50 of human Eukaryotic Translation Initiation Factor 3 Subunit L using the numbering given in entry Q9Y262.1 (GeneID 51386).
Additional Product Information
Eukaryotic translation initiation factor 3 subunit L (EIF3L) is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9Y262].
Alternate Names
eIEF associated protein HSPC021; EIF3EIP; eIF3l; EIF3S11; EIF3S6IP; eukaryotic translation initiation factor 3 subunit 6-interacting protein; eukaryotic translation initiation factor 3 subunit E-interacting protein; eukaryotic translation initiation factor 3 subunit L; HSPC021; HSPC025; MSTP005
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.