Rabbit anti-eIF4G1/eIF4GI Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-774A maps to a region between residue 200 and 250 of human eukaryotic translation initiation factor 4 gamma, 1 using the numbering given in entry NP_886553.2 (GeneID 1981).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to eIF4G1/eIF4GI immobilized on solid support.
The epitope recognized by A301-774A-T maps to a region between residue 200 and 250 of human eukaryotic translation initiation factor 4 gamma, 1 using the numbering given in entry NP_886553.2 (GeneID 1981).
Additional Product Information
Eukaryotic initiation factor 4 gamma 1 (eIF4G1) is one of 2 eIF4G proteins found in human (eIF4G1 and eIF4G3). eIF4G is a subunit of the eIF4F heterotrimeric complex composed of eIF4A, eIF4E, and eIF4G. eIF4F plays a role in recruiting the ribosome to the 5’-end of capped mRNAs during protein synthesis. In the eIF4F complex, eIF4G functions as a scaffold to bridge the cap-binding subunit, eIF4E, with the RNA helicase, eIF4A.
Alternate Names
EIF4F; EIF4G; eIF-4G 1; EIF-4G1; EIF4-gamma; eIF-4-gamma 1; EIF4GI; eucaryotic translation initiation factor 4G; eukaryotic translation initiation factor 4 gamma 1; P220; PARK18
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.