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Rabbit anti-ELYS Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
ELYS
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 2216 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to ELYS immobilized on solid support.

The epitope recognized by A300-166A maps to a region between residues 2220 and the C-terminus (residue 2302) of human ELYS transcription factor-like protein TMBS62 using the number given in entry AAN65622.1 (GeneID 25909)

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

ELYS was identified as a protein that co-purified with the Nup-107-160 nuclear pore complex. ELYS has been shown to localize to nuclear pores and to kinetochores and is required for nuclear pore assembly and proper cytokinesis.

25909
AHCTF1
AT-hook containing transcription factor 1
Q8WYP5
Protein ELYS

Alternate Names

ELYS; ELYS transcription factor-like protein TMBS62; embryonic large molecule derived from yolk sac; MST108; MSTP108; protein ELYS; Protein MEL-28; putative AT-hook-containing transcription factor 1; TMBS62

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.