Rabbit anti-Emerin Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-491A maps to a region between residue 1 to 50 of human Emerin using the numbering given in entry NP_000108.1 (GeneID 2010).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Emerin immobilized on solid support.
The epitope recognized by A304-491A-T maps to a region between residue 1 to 50 of human Emerin using the numbering given in entry NP_000108.1 (GeneID 2010).
Additional Product Information
Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene [taken from NCBI Entrez Gene (Gene ID: 2010)].
Alternate Names
EDMD; emerin; LEM domain containing 5; LEMD5; STA
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.