Rabbit anti-EPB41L2/4.1G Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-425A maps to a region between residue 575 and 625 of human erythrocyte membrane protein band 4.1-like 2 (Generally expressed protein 4.1) using the numbering given in entry NP_001422.1 (GeneID 2037).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to EPB41L2/4. 1G immobilized on solid support.
The epitope recognized by A301-425A-T maps to a region between residue 575 and 625 of human erythrocyte membrane protein band 4.1-like 2 (Generally expressed protein 4.1) using the numbering given in entry NP_001422.1 (GeneID 2037).
Additional Product Information
EPB41L2/4.1G is a member of the protein 4.1 family which includes erythroid protein 4.1 (4.1R), 4.1N (neuronal), 4.1B (brain) and 4.1G (general). The protein 4.1 family members are defined by the presence of a FERM, SAB, and CTD domain and play a role in cell membrane and cytoskeletal structural organization. EPB41L2/4.1G is widely expressed and localizes to the cytosol, perinuclear area, and centrosomes.
Alternate Names
4.1G; 4.1-G; band 4.1-like protein 2; erythrocyte membrane protein band 4.1 like-protein 2; generally expressed protein 4.1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.