Rabbit anti-EPS8L2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-842A maps to a region between residue 665 and 715 of human epidermal growth factor receptor pathway substrate 8-like protein 2 using the numbering given in entry NP_073609.2 (GeneID 64787).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to EPS8L2 immobilized on solid support.
The epitope recognized by A301-842A-T maps to a region between residue 665 and 715 of human epidermal growth factor receptor pathway substrate 8-like protein 2 using the numbering given in entry NP_073609.2 (GeneID 64787).
Additional Product Information
EPS8L2 (epidermal growth factor receptor kinase substrate 8-like protein 2) bears significant similarity to eps8. Like eps8, EPS8L2 has been shown to interact with SOS-1, a guanine nucleotide exchange factor (GEF), and activate its Rac-GEF activity. EPS8L2 localizes to PDGF-induced F-actin rich ruffles and plays a role in mediating receptor-tyrosine kinase activated actin remodeling.
Alternate Names
DFNB106; epidermal growth factor receptor kinase substrate 8-like protein 2; epidermal growth factor receptor pathway substrate 8-related protein 2; EPS8-like protein 2; EPS8R2; EPS8-related protein 2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.