Rabbit anti-ErbB3 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-370A maps to a region between residue 1292 to 1342 of human V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 using the numbering given in entry NP_001973.2 (GeneID 2065).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to ErbB3 immobilized on solid support.
The epitope recognized by A304-370A-T maps to a region between residue 1292 to 1342 of human V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 using the numbering given in entry NP_001973.2 (GeneID 2065).
Additional Product Information
ErbB3, also known as HER3, is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors[taken from NCBI Entrez Gene (Gene ID: 2065)].
Alternate Names
c-erbB3; c-erbB-3; ErbB-3; erbB3-S; HER3; human epidermal growth factor receptor 3; LCCS2; MDA-BF-1; p180-ErbB3; p45-sErbB3; p85-sErbB3; proto-oncogene-like protein c-ErbB-3; receptor tyrosine-protein kinase erbB-3; tyrosine kinase-type cell surface receptor HER3; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.