Rabbit anti-Erbin Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-763A maps to a region between residue 825 and 875 of human Erbb2 Interacting Protein using the numbering given in entry EAW51335.1 (GeneID 55914).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Erbin immobilized on solid support.
The epitope recognized by A303-763A-T maps to a region between residue 825 and 875 of human Erbb2 Interacting Protein using the numbering given in entry EAW51335.1 (GeneID 55914).
Additional Product Information
Erbin (Erbb2-interacting protein) is a member of the leucine-rich repeat and PDZ domain (LAP) family. Erbin contains 17 leucine-rich repeats and one PDZ domain. It binds to the unphosphorylated form of the ERBB2 protein and regulates ERBB2 function and localization. It has also been shown to affect the Ras signaling pathway by disrupting Ras-Raf interaction [taken from NCBI Entrez Gene (Gene ID: 55914)].
Alternate Names
densin-180-like protein; epididymis secretory protein Li 78; Erbb2-interacting protein; ERBB2IP; erbin; HEL-S-78; LAP2; protein LAP2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.