Rabbit anti-ERK5 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-656A maps to a region between residue 766 and 816 of human extracellular-signal-regulated kinase 5 using the numbering given in entry NP_002740.2 (GeneID 5598).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to ERK5 immobilized on solid support.
The epitope recognized by A302-656A-T maps to a region between residue 766 and 816 of human extracellular-signal-regulated kinase 5 using the numbering given in entry NP_002740.2 (GeneID 5598).
Additional Product Information
ERK5 (extracellular signal-regulated kinase 5) is a member of the MAP kinase family. ERK5 is also known as MAPK7 (mitogen-activated protein kinase 7). ERK5 is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors [taken from NCBI Entrez Gene (GeneID: 5598)].
Alternate Names
big MAP kinase 1; BMK1; BMK-1; BMK1 kinase; ERK4; ERK5; ERK-5; Extracellular signal-regulated kinase 5; extracellular-signal-regulated kinase 5; MAP kinase 7; MAPK 7; mitogen-activated protein kinase 7; PRKM7
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.