Rabbit anti-Exo70 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-365A maps to a region between residue 1 and 50 of human Exocyst Complex Component Exo70 using the numbering given in entry NP_001138769.1 (GeneID 23265).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Exo70 immobilized on solid support.
The epitope recognized by A303-365A-T maps to a region between residue 1 and 50 of human Exocyst Complex Component Exo70 using the numbering given in entry NP_001138769.1 (GeneID 23265).
Additional Product Information
Exo70 is a component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. In adipocytes, Exo70 plays a crucial role in targeting SLC2A4 vesicle to the plasma membrane in response to insulin, perhaps directing the vesicle to the precise site of fusion [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9UPT5].
Alternate Names
2-5-3p; BLOM4; EX070; EXO70; Exo70p; EXOC1; exocyst complex component 7; exocyst complex component Exo70; YJL085W
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.