Rabbit anti-EZH2 Recombinant Monoclonal Antibody [BLR338M]
Product Details
Specifications
Immunogen was a recombinant protein representing human Histone-lysine N-methyltransferase EZH2 (residues 1-154, NP_004447.2).
Additional Product Information
Enhancer of zeste homolog 2 (EZH2) is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. EZH2 may play a role in the hematopoietic and central nervous systems [taken from NCBI Entrez Gene (Gene ID: 2146)].
Alternate Names
enhancer of zeste homolog 2; ENX1; ENX-1; EZH2b; histone-lysine N-methyltransferase EZH2; KMT6; KMT6A; lysine N-methyltransferase 6; WVS; WVS2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (A120-113P) with 5% Normal Pig Serum (S100-020) added to the blocking buffer.