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Rabbit anti-FCHO2 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
FCHO2
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 300 and 350
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Presumed Reactivity
Mouse, Rat
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to FCHO2 immobilized on solid support.

The epitope recognized by A304-560A maps to a region between residue 125 to 175 of human FCH Domain Only 2 using the numbering given in entry NP_620137.2 (GeneID 115548).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to FCHO2 immobilized on solid support.

The epitope recognized by A304-560A-T maps to a region between residue 125 to 175 of human FCH Domain Only 2 using the numbering given in entry NP_620137.2 (GeneID 115548).
Country of Origin
USA

Additional Product Information

FCH domain only protein 2 (FCHO2) may play a role in membrane remodeling by imposing and stabilizing particular membrane curvatures [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q0JRZ9].

115548
FCHO2
FCH domain only 2
Q0JRZ9
F-BAR domain only protein 2

Alternate Names

F-BAR domain only protein 2; FCH domain only protein 2

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.