Rabbit anti-Fen1 IHC Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by IHC-00335 maps to a region between residues 250 and 300 of human Flap Structure Endonuclease 1 using the numbering given in entry NP_004102.1 (GeneID 2237).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Fen1 immobilized on solid support.
The epitope recognized by IHC-00335-T maps to a region between residues 250 and 300 of human Flap Structure Endonuclease 1 using the numbering given in entry NP_004102.1 (GeneID 2237).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Flap endonuclease 1 (Fen1) is a member of the XPG/RAD2 endonuclease family. Fen1 functions primarily as an endonuclease that removes 5’-overhangs during base-excision DNA repair and lagging strand DNA synthesis. For these processes Fen1 interacts with PCNA and the 9-1-1 DNA repair complex. Fen1 also possesses 5’ to 3’ exonuclease activity as well as RNase H activity.
Alternate Names
DNase IV; FEN-1; flap endonuclease 1; Flap structure-specific endonuclease 1; hFEN-1; Maturation factor 1; maturation factor-1; MF1; RAD2
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Overnight incubation at room temperature is recommended.
Likely to work with frozen sections.