Rabbit anti-FIP1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-461A maps to a region between residue 1 and 50 of human factor interacting with PAP using the numbering given in entry NP_112179.2 (GeneID 81608).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to FIP1 immobilized on solid support.
The epitope recognized by A301-461A-T maps to a region between residue 1 and 50 of human factor interacting with PAP using the numbering given in entry NP_112179.2 (GeneID 81608).
Additional Product Information
FIP1 was originally identified as Rhe (rearranged in hypereosinophilia) and part of a fusion protein encoded by a genetic rearrangement between the N-terminal region of FIP1 (Rhe) and the C-terminal region of the platelet-derived growth factor receptor alpha (PDGFRalpha). The fusion protein exhibits aberrant tyrosine kinase activity and is associated with hypereosinophilic syndrome (HES), a rare hematologic disorder characterized by an overproduction of eosinophils that results in organ damage. FIP1 was also identified as FIP1-like 1 in a database search for a human homolog of the yeast Fip1p (factor interacting with PAP), a subunit of the yeast 3’-end processing complex, CPF (cleavage and polyadenylation factor). Human FIP1 was similarly found to interact with PAP and CPSF160 as part of the human CPSF (cleavage and polyadenylation specificity factor) complex. In association with CPSF160, FIP1 is able to recognize U-rich elements on pre-mRNA and stimulate PAP activity.
Alternate Names
factor interacting with PAP; FIP1; FIP1 like 1; FIP1L1 cleavage and polyadenylation specific factor subunit; FIP1-like 1 protein; hFip1; pre-mRNA 3'-end-processing factor FIP1; rearranged in hypereosinophilia; Rhe
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.