Rabbit anti-FKBP-135 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-211A-T maps to a region between residues 1175 and the C-terminus (residue 1234) of human FK506-binding protein 135 using the numbering given in TrEMBL entry Q9Y4D0 (GeneID 23307).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to FKBP-135 immobilized on solid support.
The epitope recognized by A300-211A maps to a region between residues 1175 and the C-terminus (residue 1234) of human FK506-binding protein 135 using the numbering given in TrEMBL entry Q9Y4D0 (GeneID 23307).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
FKBP-135 has been identified as protein that interacts with the macrolide immunosuppressant, FK506, isolated from streptomyces tsukubaensis. FKBP-135 is a peptidyl prolyl cis-trans isomerase involved in various cellular activities including protein folding.
Alternate Names
133 kDa FK506-binding protein; 133 kDa FKBP; FK506 binding protein 15, 133kDa; FK506-binding protein 133kDa; FK506-binding protein 15; FKBP133; FKBP-133; FKBP-15; KIAA0674; PPP1R76; protein phosphatase 1, regulatory subunit 76; WAFL; WASP- and FKBP-like protein; WASP and FKBP-like protein
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.