Rabbit anti-FNIP1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-767A maps to a region between residue 775 to 825 of human Folliculin-interacting protein 1 using the numbering given in entry Q8TF40.3 (GeneID 96459).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to FNIP1 immobilized on solid support.
The epitope recognized by A305-767A-T maps to a region between residue 775 to 825 of human Folliculin-interacting protein 1 using the numbering given in entry Q8TF40.3 (GeneID 96459).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Folliculin-interacting protein 1 (FNIP1) binds to the tumor suppressor protein folliculin and to AMP-activated protein kinase (AMPK). FNIP1 participates in the regulation of cellular metabolism and nutrient sensing by modulating the AMPK and target of rapamycin signaling pathways. FNIP1 has a closely related paralog that encodes a protein with similar binding activities. Both related proteins also associate with the molecular chaperone heat shock protein-90 (Hsp90) and negatively regulate its ATPase activity and facilitate its association with folliculin [taken from NCBI Entrez Gene (Gene ID: 96459)].
Alternate Names
folliculin-interacting protein 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.