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Rabbit anti-gamma-H2AX Recombinant Monoclonal Antibody [BLR053F]

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
gamma-H2AX
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Recombinant Monoclonal
Clone:
BLR053F
Format:
Whole IgG
Immunogen:
Surrounding Serine 140
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Purified
100 µl (50+ tests)

10 µl (5+ tests)

100 µg (BSA-Free)

$136.00 $851.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse, Human
Antigen Species
Human
Concentration
100 µg/ml 1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Request Formulation Change Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Request Formulation Change
Production Procedures
Recombinant antibody was purified from cell culture supernatant.

Immunogen was a peptide surrounding phosphorylated serine 140 of human histone H2AX using the numbering given in entry NP_002096.1 (Gene ID 3014).
Country of Origin
USA

Additional Product Information

H2AX is a member of the histone H2A family. The four core histones involved in the formation of the nucleosome structure of compacted chromatin are H2A, H2B, H3, and H4. H2AX may function to facilitate DNA repair, and recent studies have shown that H2AX is required for the maintenance of genomic stability. Gamma H2AX is the phosphorylated form of H2AX that results in response to DNA damage.

3014
H2AFX
H2A histone family member X
P16104
Histone H2AX

Alternate Names

H2A.X; H2A/X; H2AX; H2AX histone; histone H2A.x; histone H2AX

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.