Rabbit anti-Gamma-synergin Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-567A maps to a region between residue 975 to 1025 of human Gamma-synergin using the numbering given in entry NP_009178.3 (GeneID 11276).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Gamma-synergin immobilized on solid support.
The epitope recognized by A304-567A-T maps to a region between residue 975 to 1025 of human Gamma-synergin using the numbering given in entry NP_009178.3 (GeneID 11276).
Additional Product Information
Gamma-synergin interacts with the gamma subunit of AP1 clathrin-adaptor complex. The AP1 complex is located at the trans-Golgi network and associates specific proteins with clathrin-coated vesicles. Gamma-synergin may act to connect the AP1 complex to other proteins [taken from NCBI Entrez Gene (Gene ID: 11276)].
Alternate Names
adaptor-related protein complex 1 gamma subunit-binding protein 1; AP1 gamma subunit binding protein 1; AP1 subunit gamma-binding protein 1; AP1GBP1; gamma-synergin; synergin gamma; SYNG
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.